Titration Calorimetry Standards and the Precision of Isothermal Titration Calorimetry Data

Biological Applications of Isothermal Titration Calorimetry

     Most of the biological phenomena are influenced by intermolecular recognition and interaction. Thus, understanding the thermodynamics of biomacromolecule ligand interaction is a very interesting area in biochemistry and biotechnology. One of the most powerful techniques to obtain precise information about the energetics of (bio) molecules binding to other biological macromolecules is isoth...

Metal ions binding study on human growth hormone by isothermal titration calorimetric method

The interaction of hGH with some metal ions ( ) at 27°C in NaC1 solution, 50 mM was studied using Isothermal titration calorimetry. There is a set of three identical and non-interacting binding sites for binding of all these metal ions, expect . The intrinsic association equilibrium constants () are not very different for  and , and also their molar enthalpies of binding (KJ/mol for  and  KJ/mo...

Isothermal Titration Calorimetry and Molecular Dynamics Simulation Studies on the Binding of Indometacin with Human Serum Albumin

Human serum albumin (HSA) is the most abundant protein in the blood plasma. Drug binding to HSA is crucial to study the absorption, distribution, metabolism, efficiency and bioavailability of drug molecules. In this study, isothermal titration calorimetry and molecular dynamics simulation of HSA and its complex with indometacin (IM) were performed to investigate thermodynamics parameters and th...

Characterization by isothermal titration calorimetry of the interaction of coumarin derivatives, phenoxathiin and atenolol with cyclodextrins. 3) Characterization by isothermal titration calorimetry of the interaction of coumarin derivatives with human serum albumin

Application of isothermal titration calorimetry in the biological sciences: things are heating up!

BioTechniques 885 During the last 15 years isothermal titration calorimetry (ITC) has come of age. There are now in excess of 2000 instruments sited in laboratories in more than 40 countries around the world. Research scientists in such diverse fields as biophysics, cell biology, pharmaceutical screening, and food research routinely investigate their systems of interest using ITC. Why is it tha...